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NeoScope II Benchtop SEM - Come and Try It

Bring your samples and come and try Nikon/JEOL's very popular NeoScope II benchtop SEM on our booth at ACMM24 next week. The intuitive interface makes this powerful instrument as easy to operate and use as a digital camera.

Andrew Marshall, our NeoScope product expert, will be on the booth to demonstrate the many features of this instrument and be conducting seminars at 1pm and 4pm on Monday and Tuesday.

Download the brochure.
Image right: Pollen, Vac-High, 10kV, 800x

Universal Illumination System

Also available for viewing on our booth is the new generation pE-4000 LED illumination system from CoolLED. The pE-4000 sets the standard for fluorescence microscopy, with 16 selectable LED wavelengths that can be matched to most filters within and most fluorophores used with any fluorescence microscope. It provides the broadest spectrum of illumination available and also the most flexible. It can be operated as a simple on/off white illumination system (with intensity control), through to advanced control for individual settings.

Download the brochure.

Innovative Materials Characterisation Systems

EDAX will be running a series of seminars (20mins) throughout the week covering several topics:

SDD with Silicon Nitride Window - Advancements in the Element/Octane Elite SDD Series take detector technology to the next level. Silicon nitride window offers remarkable improvements in low energy sensitivity. Monday and Thursday 1pm, Tuesday 4pm.

Neighbour Patterning Average Rescanning - offers increased indexing rate dramatically, even for very noisy patterns, revealing new detail previously unseen. Monday 4pm, Wednesday 1pm.

Pattern Region of Interest Analysis System - an innovative method to use the EBSD camera and phosphor screen synergistically as both an EBSD pattern collection detector and an array of positional electron detectors. Tuesday 1pm, Wednesday 4pm.

Read the full details.

Dual-View Inverted Selective Plane Illumination Microscopy: diSPIM

The benefits of Selective Plane Illumination Microscopy, SPIM, have been acknowledged for some time now. These include reduced photo-bleaching and high acquisition speeds.

In SPIM, often referred to as lightsheet microscopy, a sheet of light is used to define a focal volume. This “lightsheet’ selectively scans through a sample with the fluorescence being detected perpendicular to the sheet of light.

Despite the benefits of SPIM over existing techniques, limitations exist which limit the applicability of SPIM. These include; sample preparation and fixing. That is, the sample needs to be mounted in a specific holder at a specific orientation which limits the technologies ability to say live-cell imaging samples on a glass coverslip, and the axial resolution, as with most imaging techniques, is compromised compared to lateral resolution.

A recent development by Dr. Hari Shroff of the NIH brings a novel development to SPIM to address the above shortcomings of the SPIM technology.

Dr. Shroff’s group has developed so-called Dual-View inverted Selective Plane Illumination Microscopy, or diSPIM. View the video on You Tube.

In diSPIM, a two objective lenses are used such that one lens generates the so-called lightsheet whilst a second lens positioned at 90° collects the fluorescence. A galvanometer is used to scan the lightsheet through the sample which can be mounted on a glass coverslip on an inverted microscope.

The limitations of axial resolution are addressed by reversing the architecture. That is, switching the objective lenses used for generating the lightsheet and fluorescence collection.

Applied Scientific Instrumentation, ASI, offer a range of systems solutions for both iSPIM (inverted selective plane illumination microscopy) and diSPIM.

Coherent Scientific is co-sponsoring a diSPIM workshop at the upcoming ACMM meeting in Melbourne. At that workshop, Dr. Hari Shroff will take participants through the benefits of iSPIM and diSPIM and specifically demonstrate the building a diSPIM microscope.

Ben Hibbs, of Coherent Scientific will be attending the workshop and we encourage you to speak with Ben regarding applications where diSPIM maybe beneficial in your laboratory.

Nikon's Powerful Advanced Inverted Microscope

The Eclipse Ti-E inverted research microscope is at the heart of the most advanced bioscience imaging protocals offering improved system speed, increased flexibility and efficient multi-mode microscopy as part of a fully-integrated microscope system that is ideal for high-end research and live cell imaging. The Ti-E is equipped with a unique Perfect Focus System (PFS) that automatically corrects focus drift in real time during a prolonged period of time-lapse imaging and also corrects focus drift in a broader range of Z-axis planes than ever before.

Image Right : Live imaging of primary rat cortical neurons stained with Hoechst33342 and DIR, photo courtesy of Ippei Kotera, Shinya Hosaka and Takeharu Nagai, Research Institute for Electronic Science, Hokkaido University

Staff

Tony Bradshaw
Microscopy - ACT/NSW, NZ
(0419) 819 259 Email
Ben Hegarty
Microscopy - NSW (Metro)
(0410) 106 100 Email
Kieren McHugh
Microscopy - QLD
(0408) 744 166 Email
Paul Pearce
Microscopy - QLD
(0401) 278 860 Email
Andrew Marshall
Microscopy - NT, SA
(0418) 833 855 Email
Peter Langford
Microscopy - TAS
(0417) 572 878 Email
Rishi Raj
Microscopy - VIC
(0421) 545 661 Email
Ben Hibbs
Laser Microscopy - QLD, VIC
(0417) 117 881 Email
Brad Hope
Microscopy - WA
(0402) 956 881 Email
Melanie Kennerley
Microscopy Support NZ
(021) 480 044 Email
Dr Robert Woolley
Laser Microscopy Specialist
(021) 288 8636 Email
Tim Watts
WPI Sales
(08) 8150 5289 Email
Julia King
National Sales Administration
(08) 8150 5255 Email
Andrew Masters
Manager
(0439) 819 095 Email

Coherent Scientific Pty. Ltd., Inc. in South Australia, Established in 1989, ABN 20 008 265 969

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