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WPI Cell Culture Cup Chambers

WPI Cell Culture Cup Chambers

WPI Cell Culture Cup Chambers

For TEER measurement of epithelial and endothelial cell cultures

World Precision Instruments

Using WPI’s EVOM2™ resistance meter, Endohm chambers provide reproducible resistance measurements of endothelial and epithelial monolayers in culture cups. Transfer cups from their culture wells to the Endohm chamber for measurement rather than using hand-held electrodes. The chamber and the cap each contain a pair of concentric electrodes: a voltage-sensing silver/silver chloride pellet in the centre plus an annular current electrode. The height of the top electrode can be adjusted to fit cell culture cups of different manufacture.

Make more precise measurements with Endohms

Endohm’s symmetrically opposing circular disc electrodes, situated above and beneath the membrane, allow a more uniform current density to flow across the membrane than with STX2 electrodes. The background resistance of a blank insert is reduced from 150Ω (when using WPI’s hand-held STX2 electrodes) to less than 5Ω. With Endohm’s fixed electrode geometry, variation of readings on a given sample is reduced from 10-30Ω with STX2 electrodes (depending on the experience of the user) to 1-2Ω. Compared with other resistance measurement methods, Endohm with EVOM2™ offers a much more convenient and economic solution to “leaky tissue” measurement. Because of the uniform density of the AC square wave current from EVOM2™, errors owing to electrode polarisation or membrane capacitance are largely eliminated. Endohm together with EVOM2™ offers the most accurate and economical endothelial ohmmeter now available. To date, cups from Corning, Millipore, Nunc, Greiner and BD Falcon have been tested. Endohm chambers may be sterilised with EtO, alcohol or a bactericide; not autoclavable.

For further information please contact us or download the datasheet.

                                                                

Endohm chamber datasheet                                                            2017 WPI catalogue (online)