Confocal.nl Re-scan Confocal Microscope

Re-scan Confocal Microscopy (RCM) is a new super-resolution technique, based on standard confocal microscopy. It’s extended with an optical re-scanning unit that projects the image directly on a CCD- or sCMOS camera. By using a sensitive camera as detector, the signal-to-noise ratio of the RCM is 4 times higher than in standard PMT-based confocal microscopes. This new technology improves lateral resolution and strongly increases sensitivity, while maintaining the sectioning capability of a standard confocal microscope. Closing down the pinhole is no longer necessary to increase resolution thanks to the re-scan step.

It is excellent for biological applications where a combination of super-resolution and high-sensitivity is required.

RCM1

With RCM1, you get super-resolution (170nm rate) with 60 to 100x objective magnification at 488nm wavelength. It's high sensitivity allows using extremely low laser power, which makes the microscope highly suitable for live cell imaging. RMC1 can be delivered in a special near infrared (NIR) version using 640nm and 785nm lasers.

• Super-resolution with very low phototoxicity
• API integration into third party software
• VIS or NIR versions available

RMC2

Our digital scanner technology allows us to use bidirectional scanning to obtain super-resolution images faster (170nm raw). RCM2 is equipped with bigger optics, which makes it suitable to work with high NA 40x to 100x magnification objectives. A lower magnification allows for a larger field of view and brighter images, since light intensity is inversely proportional to the magnification squared. RCM2 requires even lower laser power to produce bright super-resolution images with minimal phototoxicity or photobleaching.

• Super-resolution with extremely low phototoxicity
• 2fps at 512x512 pixels
• Hardware integration into third party software

For further information please contact us or read more on Confocalnl wesbite.