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Nikon Ti2-LAPP Modular Illumination System

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Nikon Ti2-LAPP Modular Illumination System

Nikon Ti2-LAPP Modular Illumination System

Modular illumination system with ultimate flexibility and expandability

Nikon

 

 

 

 

The Nikon Ti2-LAPP system provides modular illuminators for total internal reflection fluorescence (TIRF), photoactivation/conversion, photobleaching, epi-fluorescence as well as super-resolution Microscopy (N-STORM).. Each module can be flexibly combined to build microscope systems that are optimised for individual research needs. For example, multiple TIRF modules can be incorporated into a single microscope for anisotropy experiments and fast, multi-angle TIRF imaging. Combined with the Ti2's stratum structure, up to five illumination modules can be incorporated into a single microscope (e.g. two TIRFs, a FRAP, a DMD and an Epi-FL module can all be integrated into one Ti2).

 

N-STORM Modules - Achieves a resolution 20 times greater than conventional optical microscopy
From fully-automated to streamlined and motorised, these modules enable N-STORM super-resolution imaging with the Ti2-LAPP system. Both are equipped with illumination field magnification (1x, 2x, 4x), as well as motorised alignment functions. This allows these systems to delivers incredible image resolution of approximately 20 nm, which is 10 times or greater than the limit in conventional optical microscopy.

 

DMD Module - Achieves simultaneous multipoint photoactivation
The DMD module enables photoactivation and photoconversion of a user-specified pattern and position(s), whereas the conventional FRAP unit only enables photoactivation of a single, manually-positioned spot. The DMD illumination shape, size, position and number can be freely customised using the NIS-Elements software. This capability allows researchers to optically mark a subset of cells or protein populations within a single cell or multiple cells to track their behavior.

 

Motorised H-TIRF Module
The incident angle of the laser and corresponding penetration depth of the evanescent field can be controlled via NIS-Elements software. When multiple TIRF modules are mounted (see image), the penetration depth can be independently set for each wavelength.

 

Manual TIRF Module - For observation of cell membrane dynamics and single molecules
The manual TIRF module includes a gradation ND filter (similar to the H-TIRF module), enabling even TIRF illumination across the field of view. Using high-sensitivity cameras, one can image single molecules and dynamics of proteins in and near the cell membrane using this TIRF illuminator.

 

Fully Automated H-TIRF Module
The ideal incident angle and focus of the laser for TIRF observation vary depending on specimen and observation conditions. Adjusting the incident angle and focus for achieving TIRF requires skill and experience. The H-TIRF module automatically adjusts the focus and incident angle of the laser for TIRF observation by monitoring the reflection beam. 

 

FRAP Module - For Analysis of Intracellular-protein Dynamics
With this FRAP module, photobleaching and photoactivation/conversion experiments coupled with the use of high-frame-rate, high-sensitivity cameras for detection are possible. This module can spot-illuminate a target position in the cell, providing a cost-effective means for the study of intracellular protein dynamics without the use of a point-scanning confocal microscope.

 

XY Galvano Scanning Unit - For Simultaneous Photostimulation and Confocal Imaging

The XY galvano scanning unit is a photostimulation module that allows the user to stimulate the desired area of a sample through point scanning by means of laser. When mounted on the Ti2-E together with the A1 HD25/A1R HD25 confocal microscope, it allows acquisition of confocal images of live-cell dynamics while simultaneously carrying out photostimulation within a sample.

 

For further information please contact us or download the datasheet.

                                                           
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