WPI Fluorodish Cell Culture Dish

• Optical quality glass bottom for better imaging quality
• Low sample volume for expensive chemicals
• Lowest access angle for micropipette
• Poly-D-Lysine (PDL) coating
• April 2024 - Now available with black walls for improved fluorescence live cell imaging

Exceptional Imaging Quality
WPI's FluoroDish tissue culture dishes are now available in a larger range of sizes and coatings. These polycarbonate dishes provide exceptional imaging quality for many applications requiring use of inverted microscopes such as high resolution image analysis, microinjection and electrophysical recording of fluorescent-tagged cells. Taking advantage of WPI's extensive experience with low toxicity adhesives, FluoroDish uses a specially formulated adhesive that is optically clear, durable and with extremely low toxicity. Tests by an independent laboratory have shown that the 96-hour surviving rate of embryos is 100% when kept in FluoroDish, substantially better than some other brands. The bottom glass has superior UV transmission (30% transmission at 300nm, compared to less than 7% for the most popular German glass). Stringent quality control ensures that glass thickness starts within the 0.17 +/-0.01mm range.

Excellent for Both Classical and Fluorescence Microscopy
Each WPI dish has a flat (0.17mm thick) optical quality glass bottom, allowing the use of a short objective working distance, large numerical aperture (NA), and a high magnification (up to 100x). The larger NA and higher magnification provide superior quality imaging for both classical and fluorescence microscopy. Higher effective NA yields brighter images for fluorescence and higher resolution in image analysis. The glass bottom permits the use of immersion objectives with medium such as water, glycerin or oil for the highest magnification possible. To optimise heat-exchange, WPI's glass-bottom dish is designed to be flush (flat) with the microscope stage or heating unit, therefore eliminating the air gap that exists with modified plastic dishes in which a glass cover slip has been inserted.

Materials
The 23.5 mm glass dish is available uncoated or poly-D-lysine-coated. Some cell lines (e.g., PC3 and HEK) adhere well to the uncoated glass bottom dish. The poly-D-lysine coating reportedly improves the adhesion of neuron cells. You may also apply any special coating that is best for your cell line to uncoated dishes.

For further information please contact us or download the datasheet.