WPI Fluorodish Cell Culture Dish

Get the highest quality images and video for your research with WPI's optical quality glass bottom FluoroDish™ Culture Dishes. Their optical quality glass bottom is as thin as a coverslip, which minimize the degree of distortion and excellent heat transfer without any of the autofluorescence issues so common with plastic petri dishes.

WPI offer three different styles in various sizes to suit your application. For live cell imaging, embryo research, and life science researchers working with small sample volumes, the 35mm FluoroDish™ with a 10mm well (FD3510) is ideal. Researchers working with expensive chemicals or experimental drugs have chosen the FD3510 dish as an ideal tool in their research. They are also an excellent choice for microinjection applications, because they are designed with the lowest access angle for easier insertion of a micropipette during cellular microinjection. FluoroDish™ is also available in 35mm (FD35) or 50mm (FD5040) sizes for cell culturing applications. WPI also offer coated dishes that are useful in promoting cell adhesion to culture surface. 35mm dishes coated with Collagen, Fibronectin, Poly-D-Lysine, Poly-L-Lysine, or Vitronectin are available. WPI also offer Blackwall FluoroDish™ for fluorescence microscopy and TIRF (Total Internal Reflection Fluorescence) and super-resolution microscopy.

• Optical quality glass bottom for shorter working distances, larger numerical aperture and higher magnification
• Non-fluorescent glass so you can discern weaker signals
• Allows the use of immersion objectives
• Flat bottom optimises heat exchange
• Less optical distortion and superior UV transmission
• Low cytotoxicity adhesive to ensure cell's survival
• Individually packaged and gamma sterilised
• Coated dishes available for better cell adhesion
• Blackwall dishes are available to block stray lights and reduce background noise

Exceptional Imaging Quality
WPI's FluoroDish tissue culture dishes are now available in a larger range of sizes and coatings. These polycarbonate dishes provide exceptional imaging quality for many applications requiring use of inverted microscopes such as high resolution image analysis, microinjection and electrophysical recording of fluorescent-tagged cells. Taking advantage of WPI's extensive experience with low toxicity adhesives, FluoroDish uses a specially formulated adhesive that is optically clear, durable and with extremely low toxicity. Tests by an independent laboratory have shown that the 96-hour surviving rate of embryos is 100% when kept in FluoroDish, substantially better than some other brands. The bottom glass has superior UV transmission (30% transmission at 300nm, compared to less than 7% for the most popular German glass). Stringent quality control ensures that glass thickness starts within the 0.17 +/-0.01mm range.

Excellent for Both Classical and Fluorescence Microscopy
Each WPI dish has a flat (0.17mm thick) optical quality glass bottom, allowing the use of a short objective working distance, large numerical aperture (NA), and a high magnification (up to 100x). The larger NA and higher magnification provide superior quality imaging for both classical and fluorescence microscopy. Higher effective NA yields brighter images for fluorescence and higher resolution in image analysis. The glass bottom permits the use of immersion objectives with medium such as water, glycerin or oil for the highest magnification possible. To optimise heat-exchange, WPI's glass-bottom dish is designed to be flush (flat) with the microscope stage or heating unit, therefore eliminating the air gap that exists with modified plastic dishes in which a glass cover slip has been inserted.