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AX AXR Confocal Microscope

Nikon Corporation (Nikon) is pleased to announce the release of the next generation confocal microscope series, AX and AX R. This new confocal series features a completely re-designed scan head with 8K x 8K resolution*1, ultra-high speed resonant scanning, and world’s largest*2 25 mm field of view*3. Combined with a new user-friendly interface and advanced AI-based tools, the AX/AX R confocal series is designed to enable users to acquire data faster, with an unprecedented level of detail and ease.


1. Capture more data than ever before with 8K x 8K pixel density and a 25 mm field of view

The AX and AX R enables 8192 x 8192 pixel, high-resolution images to be obtained with the world’s largest field of view (25 mm). Now, ultrafine details can be easily captured even for large specimens. This feature is available for both inverted*4 and upright*5 microscope configurations, thereby supporting a wide range of research applications and fields.


In addition, with the AX R’s high-speed resonant scanner users can acquire up to 720 frames per second at 2048 x 16 pixels. High-speed resonant scanning not only enables dynamic events to be easily captured but also reduces the amount of time required to image large, fixed specimens.

Time-lapse Z series maximum intensity projection images of a developing Drosophila embryo expressing PLC-PH::GFP (PIP2) acquired every 10 minutes for 12 hours with 2K x 1K pixels (with 25X silicone immersion objective), courtesy of Yang Hong Laboratory, Department of Cell Biology, University of Pittsburgh in collaboration with the Center for Biological Imaging. With high-speed scanning, it is possible to minimise photobleaching of the specimen even with long-term imaging.


2. Improved specimen viability for longer time-lapse imaging

The AX/AX R features detector units that are twice as sensitive as conventional models*6, with a ~30% reduction in dark current noise*7. With increased sensitivity and reduced noise, lower illumination power can be used for imaging even dim specimens, minimizing photobleaching and phototoxicity. Combined with high-speed resonant scanning which further decreases exposure time, specimen viability is greatly extended, enabling extreme, long-term time-lapse imaging.


3. A simplified, AI-driven user experience

Nikon’s NIS-Elements C imaging software allows users to easily customize the layout and experiment workflow to suit their needs. The new tool simplifies confocal acquisition by automatically determining the optimal illumination and detection settings while removes shot noise from confocal images to enable clearer images with shorter exposure times. AI-based tools for post-acquisition image processing such as makes segmentation of complex structures more efficient and reliable.

Graphical programming tools for both acquisition and analysis provide further flexibility for the user. The optional JOBS module enables complex, non-linear experiments with multiple paths and dimensions to be created with ease, including conditional workflows where subsequent acquisition parameters are based on real-time analysis results.


For further information please contact us or read more.


*1 Galvano scanner
*2 Among point scanners available on the market as of 287 Apirl 2021, according to research conducted by Nikon
*3 The diameter of the observable image in a microscope
*4 ECLIPSE Ti2-E inverted research microscope
*5 ECLIPSE Ni-E motorised upright research microscope
*6 In cases where multi-alkali PMT is selected
*7 Noise generated by the heat of the sensor itself during long exposures


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Fixed Wavelength Femtosecond Laser

Coherent Inc introduces Axon 780, the newest wavelegnth in the Axon line of compact, discrete wavelength femtosecond sources.


Axon demonstrates market leading performance and features in the same form factor as other Axon lasers, enabling plug-and-play compatibility between wavelengths at the same competitive price point. Like other Axon lasers at 920nm and 1064nm, it is perfect for 2-Photon imaging applications, especially for label free and clinical pathways. It is also ideal for a variety of other applications in nanofabrication, metrology and terahertz spectroscopy.


For further information please contact us or read more.

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New Upright Microscope

Nikon Corporation (Nikon) is pleased to announce the release of the ECLIPSE Si upright microscope, with intelligent time-saving features, an automatic shut-off mode, and an ergonomic design to reduce physical strain that can result from prolonged microscope use.


Time saving features including a Light Intensity Management function which reduces light intensity adjustment times by 40%

Nikon has developed a new feature called Light Intensity Management (LIM) which automatically records the light intensity level set for each objective. As the user switches between objectives, the previously set intensity for each objective is automatically recalled. The LIM feature can reduce the time spent on manually adjusting the light intensity when switching objectives by ~40%.

In addition to LIM, the ECLIPSE Si features thoughtfully positioned components that are designed to further streamline the workflow. The focus knobs are located on both sides of the microscope for easy access. In addition, the focus knob and the stage handle can be operated with one hand, freeing up the other hand to rotate the nosepiece and replace the specimen slide. As a result, users can more efficiently observe a large number of samples.

Furthermore, users can quickly confirm the magnification and illumination status with the integrated LCD screen at the front of the microscope. Controls are marked with easy-to-understand illustrations for intuitive and efficient operation, enhancing the overall user experience and streamlining their overall workflow.


Ergonomic design reduces physical strain

The ECLIPSE Si is ergonomically designed to allow users to make observations in a natural posture. The low, 135mm stage height minimises hand and arm movements required when changing samples. The tubes, with an inclination angle of 45 degrees, allow users to easily switch between looking through the eyepieces and checking the sample on the stage while maintaining the observation posture.

Switching between magnifications can cause eye strain from sudden changes in light intensity. The LIM feature which automatically recalls optimal light intensity settings for each magnification, prevents these sudden changes and reduces eye strain. In addition, a blue light blocking filter (optional) can be used to further reduce eye irritation.

By reducing physical strain, the ECLIPSE Si can help users stay focused during long hours of observations.


Easily configure for remote image sharing

By configuring the ECLIPSE Si with the Digital Sight 1000 microscope camera (optional), images on the microscope can be projected directly to a monitor for simultaneous observation by multiple people. The ECLIPSE Si can also be configured with the DS-Fi3 microscope camera (optional), which captures high-definition images of samples in their true colors. In addition, when connected to a PC with NIS-Elements L imaging software (optional), images can be shared with other PCs and mobile devices for virtual viewing. With its remote image sharing capabilities, the ECLIPSE Si is an ideal microscope for virtual consultation and teaching.


A variety of observation methods to meet a wide range of application needs

The ECLIPSE Si supports a variety of observation methods including bright-field, phase contrast, dark-field, simple polarizing, and diascopic fluorescence*2. An eco-friendly, high luminescent white LED is used as the illumination light source.


An Online Guide provides quick instructions on how to use the microscope

The “Online Guide” is a web-based operation manual featuring video tutorials and images showing how to use the microscope, including various observation methods and operation tips. The ECLIPSE Si is accompanied with a QR code*3 that can be attached to the microscope body. Users can simply scan the QR code with their mobile phone for quick access to the Online Guide.

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Super Resolution Microscopy Special Offer

By enabling real-time super-resolution with large field of view images, the combination of SRRF-Stream and Andor's iXon EMCCD cameras represents a highly cost effective way to unlock powerful super-resolution from conventional fluorescence microscopes.

Developed in conjunction with the Ricardo Henriques Lab at UCL, SRRF-Stream is a huge leap towards Andor's vision that super resolution microscopy should be widely and easily accessible to all.

We have the special promotion running till the end of March, that SRRF-Stream is available at no additional charge with any new iXon camera ordered before 31 March 2021. For further information please contact us or read more.

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Chemical Imaging Microscope

In a class of their own, the Spero® microscopes from DRS Daylight Solutions are the world's first and only fully integrated, wide-field spectroscopic microscopy and imaging platforms based on their broadly tunable mid-infrared quantum cascade laser technology.

The Spero-QT microscope is the second-generation platform in this new class of instruments with high-performance, label-free chemical imaging. The Spero system offers unrivalled mid-IR spectroscopy, substantially outperforming FTIR microscopes in spatial resolution, speed, and field-of-view capabilities while eliminating the need for cryogenic cooling.

The new Spero-LT is the latest model in the Spero product family. The LT offers the same high-performance speed and resolution specifications as the Spero-QT flagship model but is economically configured.

For further information please contact us or read more.

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